Fetal Bovine sera

Manufacturer: J R Scientific

US Origin Fetal Bovine Serum (FBS) is the most widely used FBS in the world. Although US Origin FBS is considered the world’s standard for quality it is never the less becoming a lower standard in the view of the OIE (World Organization for Animal Health) and other regulatory bodies. On a scale of 1-4 for geographical BSE risk (GBR), with 1 being the lowest risk and 4 being the highest risk, US FBS ranks a 3 – demonstrating a moderate to high risk that BSE exists in the USA. US Origin FBS provided by J R Scientific is of the highest quality available in the USA. It is collected using state of the art aseptic collection techniques resulting in low endotoxin and hemoglobin concentrations. 

For a full serum product listing, click here!

Fast Tissue-to-PCR Kit (#K1091) - Rapid extraction and amplification of DNA

Manufacturer: Fermentas

The Fast Tissue-to-PCR kit is a complete system for rapid extraction and amplification of genomic DNA from animal tissues.There is no need for procedures as tissue mechanical disruption, overnight digestion, phenol extraction, DNA precipitation or column purification.

The procedure involves short 10 minute incubation of the sample in the Tissue Lysis Solution supplemented with Proteinase K at room temperature. Then the Neutralization Solution T is added, and the tissue extract is ready for PCR with the Tissue Green PCR Master Mix (2X).

Feature / Benefits: 

• Fast sample lysis during 10 min incubation at room temperature
• Efficient PCR genotyping
• Direct loading of PCR product on gel
• Compatibility with various sample material: tissues from mice, fish and insects, human hair and saliva

Applications

• Genotyping
• Transgene detection
• Knockout analysis
• Sequencing

Components

• Tissue Lysis Solution
• Proteinase K
• Neutralization Solution T
• Tissue Green PCR Master Mix (2X)
• Water, nuclease-free
• Detailed Protocol

Figure 2. Multiplex PCR. 
Genomic DNA from mouse tail, ear clip and finger was extracted and amplified with Fast Tissue-to-PCR Kit. 114, 214 and 388 bp PCR products were generated using three sets of primer pairs.
M – GeneRuler™ Express DNA Ladder (#SM1551)
1, 2 – mouse ear clip
3, 4 – mouse tail
5, 6 – mouse finger

Epitope Tag Antibody – Detection of Tag-fusion proteins

Manufacturer: Nacalai Tesque, Inc

Nacalai Tesque carries an impressive variety of antibodies.Apart from a wide range of secondary antibodies, recently tag antibodies are developed in increased numbers. Currently available tagged antibody types are: GFP, c-Myc, His, V5, GST. Most of these types are available in POD coupled form as well. Uniquely, GFP (Rat IgG2a) is suitable for immunohistochemical detection!

Feature / Benefits: 

• Immunohistochemical application
• Rat monoclonal antibody

Specifications:

Signal Enhancer Hikari - Signal Enhancer for Western Blotting and ELISA

Manufacturer: Nacalai Tesque, Inc

Signal Enhancer HIKARI resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA. Witness a more effective assay with Signal HIKARI instead of conventional diluents such as PBS and TBS to detect the protein of interest.

Features:

• Enhances antigen-antibody reactions 
• Reduces background 
• Works with any substrate 
• Ready-to-use reagent 

Ordering information

 

 

Detection enhancement of Rac1 with treatment of HIKARI in Western blotting

Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

Signal Enhancer Hikari for Immunostain – Detect protein of interest with Immunostain procedures

Manufacturer: Nacalai Tesque, Inc

Signal Enhancer HIKARI for Immunostain resolves the problems of low sensitivity and high background often encountered during immunostain procedures. Signal Enhancer HIKARI produces a significant increase in the ability to detect protein of interest and eliminate unwanted background.

Feature / Benefits:

• Enhances signals 
• Reduces background
• Ready-to-use reagent
• Works with any system - applicable to colorimetric, chemiluminescent and fluorescent detection

Specifications: Product information

*Note: Signal Enhancer HIKARI for Immunostain Solution A and B exhibit different enhancing effects, depending on antigens and antibodies. These solutions can be used independently and a Trial set is available to help researchers select the suitable solution for their use.

Brain tissue section was stained with secondary antibody conjugated with AF555 (red) and counter stained with Hoechst dye (blue).

RNAGEM – Extraction of RNA from mammalian cell culture

Manufacturer: ZyGEM

RNAGem™ tissue RNA extraction kits feature a new formulation of enzymatic technologies that uses a single-tube process to provide complete extraction of RNA. This includes smaller RNA molecules such as noncoding RNA and microRNA that may be excluded by other RNA extraction methods. The RNAGem kits deliver high molecular weight RNA and good transcriptome profiles. They are suitable for complete gene-expression and noncoding RNA analyses.

Feature / Benefits:

• Takes less than 10 minutes from start to finish, including handling time
• Requires a single 5 minute incubation
• Does not use GITC, phenol, chloroform or isopropanol
• Delivers linear yields of total Nucleic Acid from 1 to 105 cells
• Delivers linearity that is matched between high and low copy genes
• Does not discriminate RNA molecules based on size

Kit Components of RNAGEM™ Tissue

• RNAGEM enzyme
• 10 x SILVER buffer
• 10 x TE storage buffer
• QuickStart Guide

jetPRIME™ DNA and/or siRNA Transfection Reagent, ONE reagent for ALL your transfection experiments

Manufacturer: Polyplus-transfection

If you routinely perform transient gene expression studies and/or gene silencing by RNA interference, the new powerful jetPRIME™ is the reagent for you!

Transfection with this versatile reagent requires low quantities of nucleic acid and small amounts of reagent. Therefore, jetPRIME™ is both gentle to cells and economical. A simple and convenient protocol ensures that it’s easy to scale up or scale down.

Features/Benefits:

• Outstanding DNA transfection efficiency
• Economical: less DNA and less reagent needed
• Better cell viability
• Excellent gene silencing
• Cotransfection of DNA & siRNA
• shRNA and miRNA plasmid transfection
• Convenient protocol

Specifications:

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request

CONTACT US for a trial size of jetPRIME™ DNA & siRNA transfection reagent. Special offer with your first order of jetPRIME™!

For more information on jetPRIME™, click here.

DNAgard™ Tissues & Cells - Stabilize DNA in tissue for at least 2 months at ambient temperature

Manufacturer: Biomatrica, Inc.

DNAgard is a new Biomatrica product for the preservation and storage of DNA in tissues and cell culture lines at room temperature. DNAgard is an aqueous, non-toxic tissue and cell storage solution that permeates cells and stabilizes intracellular DNA in unfrozen samples. A unique dry-down feature allows extended storage time for one year at room temperature. Extraction by standard techniques yields high quality DNA ready for all downstream analyses.

Find out more on DNAgard™ Tissues & Cells and other products from Biomatrica here.

DNA in vitro transfection: jetPEI™

Manufacturer: Polyplus-transfection

Figure: HEK-293 cells expressing GFP after transfection using jetPEI™.

Polyplus-transfection has developed a complete range of transfection reagents based on the properties of the linear cationic polyethylenimine (PEI) molecule*.

jetPEI™ transfection reagent, for reproducible transfection with low toxicity

Features/Benefits:

jetPEI™ is a unique, non-lipid, easy-to-use transfection reagent that isn't negatively affected by serum or even antibiotics. A distinctive linear polyethylenimine (PEI) derivative, jetPEI™ gently facilitates intracellular DNA delivery for high performance. It is able to do this without toxicity while successfully transfecting hundreds of different cell lines and primary cells Activity on a broad range of cells and an easy protocol make jetPEI™ ideal for routine and HTS applications.

For the complete jetPEI™ story, visit Polyplus-transfection jetPEI™.

Specifications:

Universal Reference Products

Manufacturer: Biochain, Inc

Universal Total RNA, cDNA and Total Protein

• From Human, Dog, Monkey, Mouse, Rat, and Chicken
• Male and Female Adults from Each Species
• Broad Representation of Genes and Proteins

Features/Benefits:

• Made from very diversified tissues for broad gene / protein coverage
• Industrial production scale to minimize lot-to-lot variation
• High quality without contamination

Search online to view BioChain’s comprehensive range of Universal Reference Products.

Search for “Universal ” @ http://www.biochain.com/

Coated Slides (Epoxysilane, Aldehydesilane, Aminosilane, or Hydrogel Coating)

Manufacturer: SCHOTT Nexterion

Nexterion® Slide E is especially suitable for covalent immobilization of amino-modified and unmodified Oligonucleotides. The epoxy surface coating allows efficient and stable attachment of the biomolecules and provides optimal probe presentation. The nucleic acids react instantly with the epoxy modified surface to form a stable covalent bond. The density of epoxy-groups of the surface remains constant over the entire surface of the slide and has been adjusted to provide optimal binding capacity. The hydrophobic surface creates smaller spot diameters ideally suited for high-density arrays. The surface chemistry is stable and remains active even during very long spotting runs.

Recommended Probe Types:

• Amino-modified and unmodified oligonucleotides
• cDNA molecules and PCR products

Features/Benefits:

• Stable and covalent binding of probes
• Superior signal-to-background ratios even from weakly expressed genes
• Hydrophobic coating supports the spotting of high-density arrays
• Epoxy chemistry enables long spotting runs

A full range of functional coating chemistries for DNA and protein microarraying is available. Please contact our Product Specialist for your specific microarray application needs.