jetPRIME™ DNA and/or siRNA Transfection Reagent, ONE reagent for ALL your transfection experiments

Manufacturer: Polyplus-transfection

If you routinely perform transient gene expression studies and/or gene silencing by RNA interference, the new powerful jetPRIME™ is the reagent for you!

Transfection with this versatile reagent requires low quantities of nucleic acid and small amounts of reagent. Therefore, jetPRIME™ is both gentle to cells and economical. A simple and convenient protocol ensures that it’s easy to scale up or scale down.

Features/Benefits:

• Outstanding DNA transfection efficiency
• Economical: less DNA and less reagent needed
• Better cell viability
• Excellent gene silencing
• Cotransfection of DNA & siRNA
• shRNA and miRNA plasmid transfection
• Convenient protocol

Specifications:

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request

CONTACT US for a trial size of jetPRIME™ DNA & siRNA transfection reagent. Special offer with your first order of jetPRIME™!

For more information on jetPRIME™, click here.

DNAgard™ Tissues & Cells - Stabilize DNA in tissue for at least 2 months at ambient temperature

Manufacturer: Biomatrica, Inc.

DNAgard is a new Biomatrica product for the preservation and storage of DNA in tissues and cell culture lines at room temperature. DNAgard is an aqueous, non-toxic tissue and cell storage solution that permeates cells and stabilizes intracellular DNA in unfrozen samples. A unique dry-down feature allows extended storage time for one year at room temperature. Extraction by standard techniques yields high quality DNA ready for all downstream analyses.

Find out more on DNAgard™ Tissues & Cells and other products from Biomatrica here.

DNA in vitro transfection: jetPEI™

Manufacturer: Polyplus-transfection

Figure: HEK-293 cells expressing GFP after transfection using jetPEI™.

Polyplus-transfection has developed a complete range of transfection reagents based on the properties of the linear cationic polyethylenimine (PEI) molecule*.

jetPEI™ transfection reagent, for reproducible transfection with low toxicity

Features/Benefits:

jetPEI™ is a unique, non-lipid, easy-to-use transfection reagent that isn't negatively affected by serum or even antibiotics. A distinctive linear polyethylenimine (PEI) derivative, jetPEI™ gently facilitates intracellular DNA delivery for high performance. It is able to do this without toxicity while successfully transfecting hundreds of different cell lines and primary cells Activity on a broad range of cells and an easy protocol make jetPEI™ ideal for routine and HTS applications.

For the complete jetPEI™ story, visit Polyplus-transfection jetPEI™.

Specifications:

Universal Reference Products

Manufacturer: Biochain, Inc

Universal Total RNA, cDNA and Total Protein

• From Human, Dog, Monkey, Mouse, Rat, and Chicken
• Male and Female Adults from Each Species
• Broad Representation of Genes and Proteins

Features/Benefits:

• Made from very diversified tissues for broad gene / protein coverage
• Industrial production scale to minimize lot-to-lot variation
• High quality without contamination

Search online to view BioChain’s comprehensive range of Universal Reference Products.

Search for “Universal ” @ http://www.biochain.com/

Coated Slides (Epoxysilane, Aldehydesilane, Aminosilane, or Hydrogel Coating)

Manufacturer: SCHOTT Nexterion

Nexterion® Slide E is especially suitable for covalent immobilization of amino-modified and unmodified Oligonucleotides. The epoxy surface coating allows efficient and stable attachment of the biomolecules and provides optimal probe presentation. The nucleic acids react instantly with the epoxy modified surface to form a stable covalent bond. The density of epoxy-groups of the surface remains constant over the entire surface of the slide and has been adjusted to provide optimal binding capacity. The hydrophobic surface creates smaller spot diameters ideally suited for high-density arrays. The surface chemistry is stable and remains active even during very long spotting runs.

Recommended Probe Types:

• Amino-modified and unmodified oligonucleotides
• cDNA molecules and PCR products

Features/Benefits:

• Stable and covalent binding of probes
• Superior signal-to-background ratios even from weakly expressed genes
• Hydrophobic coating supports the spotting of high-density arrays
• Epoxy chemistry enables long spotting runs

A full range of functional coating chemistries for DNA and protein microarraying is available. Please contact our Product Specialist for your specific microarray application needs.