DNA Methylation Detection Kit

Manufacturer: Fermentas

Recent studies indicate that DNA methylation of a cytosine located 5' to guanosine has important regulatory effects on gene expression, especially when CpG-rich areas located in the promoter regions of many genes are methylated. Studies have also shown that aberrant methylation of CpG-rich areas in the promoter regions is associated with development of human cancer.

Methylation-specific PCR (MSP) is a recently developed technique for determination of methylation patterns from very small amount of DNA samples. Sodium bisulfite treatment is the critical step involved in this technique. BioChain's DNA methylation detection kit offers a fast, efficient and convenient alternative to traditional bisulfite conversions.

Our kit provides users with a true endogenous human positive control which eliminates any ambiguity in validating results.We have optimized our protocol to minimize DNA degradation, reagent preparation, and conversion time. Our protocol guarantees > 99 % conversion of cytosine to uracil and > 99 % CpG protection, as calculated by sequencing, and is sensitive over a range of 2 µg - 500 pg of DNA. In less than 3 hours, users can obtain high quality bisulfite modified DNA ready for analysis by PCR, sequencing, or microarrays.

 

Features/Benefits:

• Fast, Efficient and a Convenient alternative to traditional bisulfite conversions
• Includes a true endogenous human positive control (Positive control has been tested in over 15 different human tissues)
• Eliminates any ambiguity in validating results.
• In less than 3 hours, users can obtain high quality bisulfite modified DNA ready for analysis by PCR, sequencing, or microarray.

Product No.	Description	Components
K5082100	DNA Methylation Detection Kit	Activator Solution (ACS)* x 500 μl Stabilizer Solution (STS)* x 500 μl Conversion Reagent (CVR)* x 5 tubes Equilibration Buffer (EQB) x 20 ml Desulfonation Buffer (DSB) x 4 ml Elution Buffer (TE) x 1.5 ml Control MetPrimers, 5 μM (CMP) x 100 μl Control Human DNA (2 μg) in 100 μl Columns x 50

 

 

Spectra™ Multicolor Protein Ladders

Manufacturer: Fermentas

Fermentas completes their range of multi-coloured SpectraTM Protein Ladders with the launch of Spectra High Range and Low Range Protein Ladders.

 

Features/Benefits:

• Sharp and stable colors.
• Multi-colour – easy to track on gel.
• Ready-to-load – no heating, further dilution or addition of a reducing agent required before use.
• Excellent Western transfer.
• Available in Broad Range, High Range and Low Range to cater to your analysis needs!

Applications:

• Monitoring of protein migration during SDS-PAGE.
• Verifying Western transfer efficiency.
• Approximate sizing of proteins on SDS-polyacrylamide gels and Western blots.
• Locating a protein of interest for excision from an unstained preparation gel.

I. Spectra™ Multicolor Broad Range Protein Ladder, #SM1841	II. NEW! Spectra™ Multicolor High Range Protein Ladder, #SM1851	III. NEW! Spectra™ Multicolor Low Range Protein Ladder, #SM1861

 

Specifications:

Cat #	Product name	Package size
SM1841	Spectra™ Multicolor Broad Range Protein Ladder	2x250 μl 50 mini gel appl, 10 µl/well 25 large gel appl, 20 µl/well
SM1842	Spectra™ Multicolor Broad Range Protein Ladder	10x250 μl 250 mini gel appl, 10 µl/well 125 large gel appl, 20 µl/well
SM1851	Spectra™ Multicolor High Range Protein Ladder	2x250 μl 50 mini gel appl, 10 µl/well 25 large gel appl, 20 µl/well
SM1861	Spectra™ Multicolor Low Range Protein Ladder	1x250 μl 50 mini gel appl, 5 µl/well 25 large gel appl, 10 µl/well

 

 

Signal Enhancer HIKARI

Manufacturer: Nacalai Tesque, Inc

Signal Enhancer HIKARI is for Western Blotting, dot blotting and ELISA. It improves antigen-antibody reactions. It resolves the problems of low sensitivity and high background.

Simply dilute antibodies with Signal Enhancer instead of diluents such as PBS or TBS. No additional steps are required!

 

Features/Benefits:

• Enhances antigen-antibody reactions
  Increases signals several times to several tens of times higher
  
  
• Easily removes background
  Increases the specificity of your antibodies
  
  
• Works with any substrate
  Enhances both chemiluminescence and colorimetric detection
  
  
• Works with any membrane
  Enhances signal on nitrocellulose and PVDF membrane
  
  
• Ready-to-use
  Just dilute your antibodies with Signal Enhancer HIKARI. No additional steps are required
  

 

Specifications:

Please call our Product Specialist for more detailed information.