Products - Genemachines HydroShear

Manufacturer: Genomic Solutions Inc.

Description:

The HydroShear offers the simplest, most reproducible, and most controllable method available for generating random DNA fragments. This software-driven device uses hydrodynamic shearing forces to fragment DNA strands.

With the HydroShear, virtually any source of DNA at any concentration in volumes as small as 40µl can be randomly fractured to within a two-fold size distribution. For instance, if your target size is 2kb, you can calibrate the HydroShear to produce a distribution centered on 2kb with 90% of your DNA falling between 1.3 kb and 2.6kb -- ideal for plasmid sequencing libraries. Furthermore, because of the repeatability of the HydroShear process, the distribution will be the same each day even with multiple users.

Features/Benefits:

High cloning efficiency of processed DNA
Small volume: 40µl samples can be sheared directly without dilution, perfect for precious DNA samples
Tight "two-fold" size distributions,*
Consistency regardless of DNA concentration or size, independent of user
Easy, fast operation: load DNA, run system, and wash in approximately 10 minutes

Specifications:

The HydroShear uses hydrodynamic shearing forces to fragment DNA strands. The physical mechanism is fairly straightforward:

DNA in solution is passed through a tube with an abrupt contraction.
As it approaches the contraction, the fluid accelerates to maintain the volumetric flow rate through the smaller area of the contraction.
During this acceleration, drag forces stretch the DNA until it snaps. The DNA fragments until the pieces are too short for the shearing forces to break the chemical bonds. The flow rate of the fluid and the size of the contraction determine the final DNA fragment sizes.

The system includes;

A base unit equipped with a 500µl syringe
Shearing control software compatible with Microsoft(R) Windows(R) 95 and NT(R)
Four #025 shearing assemblies
Power cord
RS-232 cord and connectors

The HydroShear comes with software for controlling and monitoring the shearing process. The software enables you to enter or read in shearing data parameters, specify a wash scheme, direct the shearing process automatically or manually monitor the current status.

With the new HydroShear Wash Kit, it removes any trace of previous samples, eliminating carryover contamination. At the same time, the kit allows you to minimize batch-to-batch variation. Complete with three different wash solutions, the HydroShear wash kit includes all reagents necessary to perform DNA shearing using the HydroShear.

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