Products - Transfection Reagents ExGen

Manufacturer: Fermentas

Description:

ExGen 500 consists of linear polyethylenimine (PEI) molecules (22kDa). These molecules have a high cationic-charge density potential which make them excellent DNA condensing and gene-delivering agents. Every third atom of the ExGen 500 backbone is a protonable amino nitrogen atom, which makes the polymeric association an effective "proton sponge". This property allows for endosome buffering and protects DNA from lysosomal degradation.

ExGen 500 Transfection reagent is available for both in vitro and in vivo transfection reactions.

Principal

As a result of charge interaction, ExGen 500 (in vitro) and DNA form complexes which settle on the cells by gravity and are absorbed by endocytosis.

In a glucose solution, ExGen 500 in vivo Transfection Reagent and DNA form cationic microprecipitates with a diameter of an approximately 50nm as a result of charge interaction. The microprecipitates attach to cellular proteoglycans and then penetrate the cell by endocytosis.

Features/Benefits:

ExGen 500 is more efficient in its ability to compact and to transfect small quantities of DNA.
Compared to other PEIs, ExGen 500 transfection efficiency is less affected by the presence of serum.Transfection can be performed in the presence or absence of serum.
ExGen 500 is suitable for the transfection of a wide number of cell types with high efficiency and low toxicity.

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FERMENTAS 2008/2009 CATALOG

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