Products - SmartMix™ HM

Manufacturer: Cepheid Inc

Description:

Ready-to-use lyophilized bead for PCR with hot start Taq polymerase, for use with Smart Cycler® systems

Features/Benefits:

Hot start functionality
This "hot start" product utilizes a Taq-specific monoclonal antibody to minimize mis-priming and/or formation of primer-dimer before thermal cycling. - Complete: OmniMix™ HS contains all of the necessary reagents for PCR, except primers and probe or intercalating dye. - Fast: No additional time required for activation. - Specific: Reduction of nonspecific product can result in improved detection.

Easy to use format
The ready-to-use format of OmniMix HS simplifies assay preparation. Simplified: Eliminating extra pipetting steps and multiple reagents reduces contamination risk and provides better reproducibility. Stable: OmniMix HS is a lyophilized bead that is stable at 2-8 °C. Scaleable: Each 0.5 mL tube contains an optimized formula of reagents for performing two 25 µL PCR amplification reactions. Larger quantities can be made by using multiple beads.

Consistency of results with OmniMix HS
Comparison of liquid reagents, individual OmniMix HS beads, and pooled OmniMix HS beads. Cycle threshold and endpoint fluorescence values obtained with a TaqMan assay incorporating a FAM-labeled probe. 100 fg of input DNA represents the limit of detection for this assay. A p value of greater than 0.05 was obtained indicating no significant difference among the three data sets

Hot start performance
Experimental results obtained with and without the use of hot start Taq polymerase. Neisseria gonorrhoeae assay with SYBR® Green and TaKaRa HS Taq polymerase or a non-hot start polymerase in liquid reagent format. Data represents the first derivative peaks from melt curve analysis immediately following 45 cycles of PCR in the presence of 0.5X SYBR Green.

Specifications:

When reconstituted to a final volume of 50 µL, OmniMix HS contains:
3U TaKaRa hot start Taq polymerase 200 µM dNTP 4 mM MgCl2 25 mM HEPES buffer pH 8.0± 0.1

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